phospho stat1 ser727 polyclonal antibody  (Proteintech)

Journal: iScience

Article Title: Resolving macrophage polarization through distinct Ca 2+ entry channel that maintains intracellular signaling and mitochondrial bioenergetics

doi: 10.1016/j.isci.2021.103339

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Article Snippet: Phospho-STAT1 (Ser727) polyclonal antibody , Thermo Fisher Scientific , Cat# PA5-97355, RRID: AB_2809157.

Techniques: Produced, Recombinant, Phagocytosis Assay, BrdU Cell Proliferation Assay, Software

Journal: Immunity

Article Title: The brain microvasculature is a primary mediator of interferon-α neurotoxicity in human cerebral interferonopathies

doi: 10.1016/j.immuni.2024.05.017

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Article Snippet: Rabbit polyclonal anti-STAT1 (Ser727) , Cell Signaling Technology , Cat#9177; RRID: AB_2197983.

Techniques: Plasmid Preparation, Recombinant, Saline, Protease Inhibitor, Western Blot, Staining, Avidin-Biotin Assay, Blocking Assay, Antibody Labeling, Reverse Transcription, Bicinchoninic Acid Protein Assay, Enzyme-linked Immunosorbent Assay, Software, Imaging

Journal: Immunologic Research

Article Title: Tryptanthrin attenuates TLR3-mediated STAT1 activation in THP-1 cells

doi: 10.1007/s12026-022-09301-z

Figure Lengend Snippet: A THP-1 Mϕ were stimulated with 20 μg/mL polyinosinic-polycytidylic acid (poly IC) for 8 h. The cell lysates were subjected to western blotting to analyze the expression of p-STAT1-Ser, p-STAT1-Tyr, and STAT1. B Cells were stimulated using 1 μg/mL lipopolysaccharides (LPS) for 4 h. The cell lysates were subjected to western blotting to analyze the expression of p-STAT1-Ser, p-STAT1-Tyr, and STAT1. C Cells were stimulated with 20 μg/mL poly IC and 0.01–1 μM tryptanthrin for 8 h. The expression of p-STAT1-Ser, p-STAT1-Tyr, and STAT1 proteins was analyzed using western blotting. Cells were stimulated with 1 μg/mL LPS and 0.01–1 μM tryptanthrin, and the expression of p-STAT1-Ser ( D ) and p-STAT1-Tyr ( E ) was analyzed at 1 h and 4 h, respectively. Representative images of three independent experiments

Article Snippet: Rabbit monoclonal anti-phosphorylated IRF3 (p-IRF3) antibody (#4947) and rabbit polyclonal anti-phosphorylated STAT1 (Ser727) (p-STAT1-Ser) antibody (#9177) were obtained from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Western Blot, Expressing

Journal: Immunologic Research

Article Title: Tryptanthrin attenuates TLR3-mediated STAT1 activation in THP-1 cells

doi: 10.1007/s12026-022-09301-z

Figure Lengend Snippet: A THP-1 Mϕ were stimulated with 20 μg/mL poly IC or 1 μg/mL LPS for 8 h or 4 h, respectively, with or without tryptanthrin. The cells were fixed, and then immunostained with anti-STAT1 antibody (red). Cell nuclei were stained with DAPI (blue). Yellow arrowhead indicates the nuclear translocation of STAT1. Representative images of three independent experiments. B THP-1 Mϕ were stimulated with 20 μg/mL poly IC or vehicle (control), and 1 μM tryptanthrin or DMSO for 8 h. Thereafter, the total RNA was extracted from the cells and expression of IFIT1 , IFIT2 , IFITM1 , ISG15 , and Mx1 mRNA was analyzed using qRT-PCR. C Similarly, after stimulating the cells with 1 μg/mL LPS or vehicle, and 1 μM tryptanthrin or DMSO for 4 h, total RNA was extracted and the mRNA expression was examined ( n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001; n.s., not significant)

Article Snippet: Rabbit monoclonal anti-phosphorylated IRF3 (p-IRF3) antibody (#4947) and rabbit polyclonal anti-phosphorylated STAT1 (Ser727) (p-STAT1-Ser) antibody (#9177) were obtained from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Staining, Translocation Assay, Control, Expressing, Quantitative RT-PCR

Journal: Immunologic Research

Article Title: Tryptanthrin attenuates TLR3-mediated STAT1 activation in THP-1 cells

doi: 10.1007/s12026-022-09301-z

Figure Lengend Snippet: A Cells were incubated with 1 μM tryptanthrin or DMSO for 24 h, and the total RNA was extracted. The mRNA expression of toll-like receptor 3 (TLR3) was examined by qRT-PCR ( n = 3; n.s., not significant). B Flow cytometry analysis of TLR3 expression in THP-1 Mϕ treated with 1 μM tryptanthrin (right panel) or DMSO (left panel). Representative histograms of three to five independent experiments. C Quantification of mean fluorescence intensity for TLR3 ( n = 3; n.s., not significant). D Cells were incubated with 1 μM tryptanthrin or DMSO for 24 h, fixed with methanol, and immunostained with anti-TLR3 antibody (green). Cell nuclei were stained using DAPI (blue). Representative images of three independent experiments. E , F Cells were stimulated with 1 ng/mL rIFN-β ( E ) or rIFN-γ ( F ) with or without tryptanthrin for up to 30 min, and the cell lysates were subjected to western blotting to analyze p-STAT1-Tyr and STAT1. G Putative mechanism of tryptanthrin-mediated regulation of TLR3 signaling in THP Mϕ. Tryptanthrin negatively modulates IRF3 activation and the subsequent IFN-β-induced phosphorylation of STAT1

Article Snippet: Rabbit monoclonal anti-phosphorylated IRF3 (p-IRF3) antibody (#4947) and rabbit polyclonal anti-phosphorylated STAT1 (Ser727) (p-STAT1-Ser) antibody (#9177) were obtained from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Incubation, Expressing, Quantitative RT-PCR, Flow Cytometry, Fluorescence, Staining, Western Blot, Activation Assay, Phospho-proteomics